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Hi Diana - I was doing some more research on Prolotherapy and ran across your question. I have a foster rescue mastiff that is going through prolotherapy right now and it is helping him. It was determined that a TTO or a TPLO would not work for him and the only thing that would would be 2 total knee replacements. The only thing I do not like is that he is put under anesthesia each time : ( . It takes him 2-3 days to fully recover and he has treatments every 2 weeks. I'm trying to research if that can be stretched out longer. He has had 4 treatments so far, along with electric acupuncture. He will never be "fixed" but he is off of all pain meds now. I don't know what else you were looking for as far as information but if you ask, I will try to answer!