Some articles you may find interesting/useful:
Puppy Shots: Vaccination Issues for Breeders
:"Conventional vaccine protocols are designed to give multiple vaccinations to puppies a few weeks apart. Most people and even many veterinarians believe that more than one vaccine is needed to "prime" the immune system or build immunity, but in the case of modified live virus vaccines for parvo and distemper, this isn't really necessary. We don't repeat vaccinations for parvo and distemper because we need vaccines more than once to form immunity. They are repeated for two basic reasons only: Habit, and to catch those few individuals who for some reason don't respond to the first vaccination. A single immunizing dose of a modified live virus vaccine - in other words, one vaccine that works - will form long term, probably lifetime, immunity to parvo and distemper. (http://www.amazon.com/exec/obidos/ASIN/0721655238/qid=1022964037/sr=1-1/caberfeidhsco-20; 2000; "Vaccines and Vaccinations: Issue for the 21st Century", Richard B. Ford and Ronald D. Schultz; (Kirk's Current Veterinary Therapy XI
, "Canine and Feline Vaccines," Phipps, Schultz; R.D. Schultz, "Considerations in Designing Effective and Safe Vaccination Programs for Dogs," May 2000; Schultz, "Duration of Immunity to Canine Vaccines: What We Know and Don't Know.")
We don't need to keep repeating the vaccines to know if they worked, either. Although titers
as a measure of ongoing immunity aren't all that useful, as a measure of whether or not an animal formed immunity from a recent vaccination, they are very reliable. ("Vaccines and Vaccinations: Issue for the 21st Century", Richard B. Ford and Ronald D. Schultz. http://www.amazon.com/exec/obidos/ASIN/0721655238/qid=1022964037/sr=1-1/caberfeidhsco-20, 2000.) Since it takes 7-10 days for the immunity to form, if you test titers ten days after a parvo and/or distemper vaccine, you will know if the puppy had an immunizing response. You don't need to guess."
Revaccination: Vaccination for Previously Vaccinated Dogs and Older Puppies
SiriusDog.com - VACCINE PROTOCOLS FOR DOGS